JISOOK KIM - RESEARCH  DOSSIER

for External Review

Research and Scholarship Activity at UTC

UTC is a primarily undergraduate institution. The Department of Chemistry and Physics offers BS degree program in Chemistry and Physics and it does not have a graduate program. My typical teaching load, per semester, includes one big lecture (Organic Chemistry for fall and Biochemistry for spring), two sections of Organic Chemistry Labs, and research courses. The number of teaching students per semester varies from 120 to 180, and I do all the grading work since we don’t have grading TAs. While managing teaching responsibility, I developed multidisciplinary research projects at the cross-disciplinary areas of bioorganic chemistry, chemical toxicology, bioinorganic chemistry, and enzymology for our undergraduate chemistry majors, which are (1) Project 1: Quinone-induced protein modifications, (2) Project 2: Interactions of metal complexes and DNA, (3) Project 3: Development of inactivators for TOPA quinone containing copper amine oxidases targeting bovine plasma amine oxidase (BPAO).

My research group at UTC, mostly based on the work of undergraduate researchers, produced total 6 published work (5 papers, 1 book chapter). 5 papers were published in peer-reviewed journals such as Bioorganic Chemistry, Inorganic Chimica Acta, and Advances in Bioscience and Biotechnology. In addition, the quality work of my research was recognized internationally, and I had an honor of contributing a book chapter titled “Biological implications of benzoquinones” (Book: “Quinone: Occurrence, Medicinal Uses and Physiological Importance”, Nova Science Publishers, Inc., 2013, ISBN 978-1-62618-323-0) with internationally known scholars together with Dr. Frederick L. Crane who is deceased at 2016. Dr. Crane is well-known for discovering coenzyme Q in the mitochondria at 1957 and is the first person who reported the discovery in the world.

For funding activity, I served as PI, Co-PI, or senior personnel for various proposals targeting NSF (MRI, RUI, REU), NIH, Research Corporation, Dreyfus, and Wheeler Foundation. External funding activity resulted in 3 funded NSF-MRI grants (total $423,376, co-PI). In addition, a recent submission to NSF MRI (PI, $392,587) was very well-received by NSF Panel/Reviewers and was recommended for funding. Surprisingly, the division director went against the Panel/Reviewers’ decision. Otherwise, this should have resulted in total amount of $815,963 from my efforts in bringing external funding support. I am in the process of writing the NSF-MRI proposal as PI for the year 2018 re-submission. Currently, I am serving as senior personnel for NSF-REU-ICompBio ($359,484, Senior personnel, pending). Internal funding activity resulted in UC Foundation Strategy Grant, (PI, $25,000, 2008), Faculty Research Fund ($3,000), Wheeler Research Fund (PI, $10,000, 2010; $10,000, 2016), and 14 Provost Student Research Awards (total $12,950 from 2008 to 2017, in an effort to help fund directed-student research projects).

For directed-student research, I have supervised 18 undergraduate researchers and 14 Provost Student Research Award projects (total: $12,950), resulting in 5 completed Honors Thesis projects (current Honors’ project not included), 4 publications with my undergraduate student coauthors, and 23 presentations at local/regional/national conferences based on my undergraduate research students’ work. Additionally, I supervised my research student's ACS Travel Award for National ACS meeting ($300, 2012).

Please click each tab to see the details for each activity. 

Published Work prior to UTC:

  1. K. Ling.; J. Kim; L. M. Sayre “Catalytic turnover of benzylamine by a model for the lysine tyrosylquinone (LTQ) cofactor of lysyl oxidase ”, J. Am. Chem. Soc., 2001, 123, 9606.
  2. C. K. Koh Choo; K. H Kim; Y. E Lee; J. S. Kim “A scientific study of Choson white ware: early porcelain from a royal kiln at Kwangju Usanni”, Archaeometry, 2002 (May), 44(2), 199.
  3. J. Kim; C. R. Wagner “Studies of the intracellular metabolism of nucleoside amino acid phosphoramidates utilizing 31P NMR and LC/MS”, Antiviral Res., 2003, 57, 47.
  4. J. Kim; D. P. Drontle; C. R. Wagner “Monitoring the intracellular metabolism of nucleoside phosphoramidate pronucleotides by 31P NMR”, Nucleosid. Nucleotid. Nucl. Acids, 2004, 23 (1), 483.
  5. J. Kim; T‑f. Chou; G. W. Griesgraber; C. R. Wagner “Direct measurement of nucleoside monophosphate delivery from a phosphoramidate pronucleotide by stable isotope labeling and LC-ESI-MS/MS”, Molecular Pharmaceutics, 2004, 1, 102.
  6. J. Kim; S­‑B. Park; N. Y. Tretyakova; C. R. Wagner “A method for quantitating the intracellular metabolism of AZT amino acid phosphoramidate pronucleotides by capillary high-performance liquid chromatography-electrospray ionization mass spectrometry”, Molecular Pharmaceutics, 2005, 2, 233.
  7. J. Kim; Y, Zhang; C, Ran; L. M. Sayre “Inactivation of bovine plasma amine oxidase by haloallylamines”, Bioorganic & Medicinal Chemistry, 2006, 14(5), 1444.

Published Work at UTC (* in bold represents undergraduate coauthor):

  1. J. Kim; A. R. Vaughn*; C. Cho*; T.V. Albu; E. A. Carver ‘Modifications of ribonuclease A induced by p-benzoquinone”, Bioorganic Chemistry, 2012, 40, 92. [PDF]
  2. Vaughn, A.R.*; Redman, C.B.*; Kang, S.M.*; Kim, J. "Biological Implications of 2-Chlorocyclohexa-2,5-diene-1,4-dione toward Ribonuclease A", Advances in Bioscience and Biotechnology, 2013, 4, 22. [PDF]
  3. J. Kim “Biological implications of benzoquinones” (Book Chapter) in the book “Quinone: Occurrence, Medicinal Uses and Physiological Importance”, Nova Science Publishers, Inc., 2013, ISBN 978-1-62618-323-0. [PDF]
  4. J. Kim; Albu, T.V.; Vaughn, A.R.*; Kang, S.M*.; Carver, E.A.; Stickle, D.M. "A comparison study on ribonuclease A modifications induced by substituted p-benzoquinones", Bioorganic Chemistry, 2015, 59, 106. [PDF]
  5. J. Kim; I. Lee “Inactivation of Bovine Plasma Amine Oxidase by 1,1,1-Trihalo-3-aminopropanes”, Bioorganic Chemistry, 2017. 75, 265. [PDF]
  6. J. Kim; A. D. Cardenal*; H. J. Greve*; W. Chen*; H. Vashi*; G. Grant; T. V. Albu “Interaction with Calf-thymus DNA and Photoinduced Cleavage of pBR322 by Rhodium(III) and Iridium(III) Complexes Containing Crown Thioether Ligands”, Inorganica Chimica Acta2018, 469, 484. [PDF]

 

External Funding Activity - Funded Proposals:

  • 2006 NSF grant (DBI-0619476) “Acquisition of a liquid chromatography mass spectrometer for biochemical and environmental Analysis” ($369,996, Co-PI, TTU)
  • 2008 NSF-MRI (0821057) “Acquisition of a Microarray Scanner and Real-Time PCR System for Interdisciplinary Research and Teaching in an Undergraduate College Setting” ($ 87,250, Co-PI) [PDF]
  • 2009 NSF-MRI (0922941) “Acquisition of a Microscopy Core System” ($184,188, Co-PI) [PDF]
  • 2010 NSF-MRI (0951711) “Acquisition of a Benchtop Single Crystal X-ray Diffractometer for the University of Tennessee at Chattanooga” ($151,938, Co-PI) [PDF]
  • 2012 ACS-CIG “Chemistry in the underprivileged regions of Chattanooga” ($400, UTC ACS Student Affiliation/Chemistry Club Advisor)
  • 2012 ACS-National Meeting ACS Travel Award “Photoinduced-DNA cleavage activity of rhodium complexes” ($300, Research Advisor; Student, Ashley Cardenal)

External Funding Activity - Pending or recommended for funding but not funded:

  • 2016 NSF-MRI (1625675) “Acquisition of a High Resolution Accurate Mass QTOF-LC Mass” ($392,735, PI, Recommended for funding by NSF Panel but not funded). [Review PDF]
  • 2017 NSF-MRI (1725527) “Acquisition of a High Resolution Accurate Mass QTOF-LC Mass” ($392,587, PI, Recommended for funding by NSF Panel but not funded). [Review PDF]
  • 2017 NSF-REU “ICompBio - Engaging Undergraduates in Interdisciplinary Computing for Biological Research” ($359,484, Senior personnel, pending)
  • 2018 NSF-MRI “Acquisition of a High Resolution Accurate Mass QTOF-LC Mass” (PI, to be submitted). 

External Funding Activity - Not funded:

  • 2007 Dreyfus “Protein modifications by the metabolites of benzene and substituted benzenes” ($30,000, PI).
  • 2008 Research Corporation “Ribonuclease Modification Induced by Substituted Benzoquinones and Hydroquinones” ($78,102, PI).
  • 2008 Research Corporation “Ribonuclease Modification Induced by Substituted Benzoquinones and Hydroquinones” ($54,936, PI).
  • 2008 NIH “Studies of protein modification induced by PAH quinones” ($150,000, PI).
  • 2009 Research Corporation “Studying the biological activity of PAH quinones using diverse fluorescence techniques” ($35,000, PI).
  • 2011 NSF-MRI (1126189) “Acquisition of a UV-Vis-Near IR Spectrofluorometer with Stopped-Flow and Fluorescence Lifetime Capabilities” ($133,314, Co-PI).
  • 2013 NIH “In-depth analysis of e-cigarette filling solutions and their biological implications” ($150,000, Co-PI).
  • 2013 NSF-RUI “Lysozyme modifications induced by substituted benzoquinones” ($223,105, PI).

Internally Funded Proposals:

  • 2007 UTC Faculty Research Grant “Investigating RNase Modification Induced by Substituted Benzoquinones and Hydroquinones” ($3,000, PI)
  • 2008 UT Foundation Strategic Initiative Grant “Biological Roles of Substituted Benzoquinones and Hydroquinones” ($25,000, PI)
  • 2010 Wheeler Research Fund “Ribonuclease A Modification Induced by the Substituted Benzoquinones” ($10,000, PI)
  • 2016 Wheeler Research Fund “Correlation study of odor intensity and chemico-biological behavior of odorous quinones” ($10,000, PI)

Internally Funded Provost Student Research Awards:

Year

Student

Title

Amount

2017

Neethu Kurien

SDS-PAGE Analysis of Naphthoquinone-Induced Protein Modification and the Effect of pH

$1,000

2014

Hendrik Greve

Fluorescence Studies of the Modification of Lysozyme by 2-Chloro-1,4-Benzoquinone

$1,000

2014

Michelle Smith

Ribonuclease A Modification Induced by 2-Hydroxy-1,4-naphthoquinone

$1,000

2013

Hendrik Greve

Fluorescence Studies on the interaction of {Ir(9S3)Cl3] with DNA

$1,000

2012

Anna Zyglewska

Electrophoretic Analysis of LysozymeModified by Chloro-benzoquinone

$1,000

2012

Ashley Cardenal

1,4-Benzoquinone: A Potential Link to Disease

$1,000

2012

Chris Cho

Fluorescence Studies on DNA Binding of [Rh(9S3)Cl₃]

$1,000

2011

Chris Cho

Kinetic Studies on the Reactions of Naphthoquinones and Amino Acids

$1,000

2011

Ashley Cardenal

The Biological Effect of PAH Quinones on Escherichia Coli

$1,000

2009

Min Kang

Investigating the Kinetics of Amino Acid Modification Induced by para-Benzoquinone Using UV-Vis Spectroscopy

$700

2009

Albert Vaughn

Monitoring Ribonuclease A (RNase A) Modification   Induced by 2-Chlorobenzoquinone Using Electrophoretic Technique

$700

2008

Caitlin Redman

Studies of Ribonuclease Modification Induced by p-Benzoquinone   Using UV/Vis Spectroscopy

$850

2008

Steve Ledford

Studies of Ribonuclease Modification Induced by Methyl para-Benzoquinone Using Electrophoresis   Technique

$850

2008

Albert Vaughn

Methodology Development for the Synthesis of a DNA Binding Agent Using an Amide Coupling Approach

$850

 

 

Below is a list of students who worked in my lab. The year represents the year of graduation. Please click ACS poster, Research Report, Thesis or Publication to view the outcome of student research for each student.

Invited Presentations: 

  • Jisook Kim “Intracellular delivery of antiviral/anticancer agents utilizing a pronucleotide approach” The Advanced Chemistry Seminar, TTU, Cookeville, TN October 2004.
  • Jisook Kim “Studying the chemical biology of small molecules”, UTC, Chattanooga, TN, August 2007.
  • Jisook Kim, “Biological Implications of Benzoquinones” at the University of the South, Sewanee, October 2014.

Student Presentations at State, Regional or National Meetings:

  • J. Kim; D. B. Thomas* “Model studies on the modification of amino acids and proteins induced by polycyclic aromatic hydrocarbon metabolites” 231st ACS National Meeting, Atlanta, GA, March 2006.
  • R. A. Patton*; J. Kim “Investigating RNase modification by 1,4-benzoquinone and 1,4-hydroquinone” 235th ACS National Meeting, New Orleans, LA, April 2008.
  • S. W. Ledford*; C. B. Redman*; A. R. Vaughn; J. Kim “Methyl-p-Benzoquinone Induced Ribonuclease a Modification” the 60th SERMACS, Nashville, TN, November 2008
  • C. B. Redman*; A. R. Vaughn*; S. W. Ledford*; J. Kim “Investigating Ribonuclease a Modification Induced by Quinones Using UV/Vis Spectroscopy” the 60th SERMACS, Nashville, TN, November 2008
  • A. R. Vaughn*; C. B. Redma*n; S. W. Ledford*; J. Kim “Ribonuclease a Modification Induced by Chloro-p-Benzoquinone” the 60th SERMACS, Nashville, TN, November 2008
  • S. W. Ledford*; C. B. Redman*; J. Kim “Methyl-p-Benzoquinone Induced Ribonuclease Modification” 237th ACS National Meeting, Salt Lake City, UT, March, 2009
  • A. R. Vaughn*; M. Kang*; J. Kim “Investigating Biological Role of Chloro-p-Benzoquinone in Ribonuclease A Modification” 237th ACS National Meeting, Salt Lake City, UT, March, 2009
  • S. W. Ledford*; C. B. Redman*; J. Kim “Methyl-p-Benzoquinone Induced Ribonuclease Modification” Celebration of Faculty Scholarship, UTC, Chattanooga, TN, April, 2009
  • A. R. Vaughn*; M. Kang*; J. Kim “Investigating Biological Role of Chloro-p-Benzoquinone in Ribonuclease A Modification” Celebration of Faculty Scholarship, UTC, Chattanooga, TN, April, 2009
  • M. Kang*; A. R. Vaughn*; J. Kim “Kinetic Studies of Amino Acid Modification Induced by PAH Benzoquinones (ID 17008)”, 237th ACS National Meeting, San Francisco, CA, March, 2010
  • A. R. Vaughn*; M. Kang*; J. Kim “Modification of Ribonuclease A Induced by 2-Chlorocyclohexa-2,5-diene-1,4-dione (ID 16998)”, 237th ACS National Meeting, San Francisco, CA, March, 2010
  • A. Cardenal*; J. Kim “Photoinduced-DNA cleavage activity of rhodium complexes”, 243rd ACS National Meeting, San Diego, CA, March, 2012
  • C. Cho*; A. R. Vaughn*; E. A. Carver; T. V. Albu; J. Kim “Studies of Ribonuclease A modifications induced by para-benzoquinone”, 243rd ACS National Meeting, San Diego, CA, March, 2012
  • H. J. Greve*, J. Kim "Studies of DNA binding interactions with [Rh(9S3)Cl3], [Rh(9S2O)Cl3], and [Ir(9S3)Cl3] using fluorescence spectroscopy" the 65th SERMACS, Atlanta, GA, November 2013
  • H. J. Greve*, J. Kim "Studies of lysozyme modifications by substituted benzoquinones" the 66th SERMACS, Nashville, TN, October 2014
  • M. D. Smith* "Ribonuclease A modifications induced by 2-hydroxy-1,4-naphthoquinone and 1,2-naphthoquinone" the 66th SERMACS, Nashville, TN, October 2014
  • Charles Thomas*, Jisook Kim, Titus Albu “Fluorescence and UV-Vis Studies of Lysozyme Modifications Induced by Quinones”, Tennessee Physiological Society Meeting, Chattanooga, October 2015
  • Charles Thomas*, Mary Booker*, Jisook Kim, Titus Albu “Fluorescence and UV-Vis studies of protein modifications induced by quinones” National ACS meeting, March 2016
  • Charles Thomas*, Mary Booker*, Jisook Kim, Titus Albu “Fluorescence and UV-Vis studies of protein modifications induced by quinones” UTC Research Dialogues, UTC, April 2016
  • Mary Booker*, Charles Thomas*, Titus Albu, Jisook Kim “Cyclic Voltammetry of Quinones in Buffered Aqueous Solution” UTC Research Dialogues, UTC, April 2016
  • Charles Thomas*, Mary Booker*, Jisook Kim, Titus Albu "Fluorescence and UV-VIS Studies of Quinone-Induced Protein Modifications", SERMACS, November 2016
  • Neethu Kurien*, Jisook Kim "Analysis of Quinone-induced Protein Modifications using SDS-PAGE", SERMACS, November 2017
  • Jake Ewald*, Jisook Kim, Titus Albu* "Fluorescence and UV-VIS Studies of Protein Modifications Induced by Quinones", SERMACS, November 2017

Graduate Student and Postdoc Presentations:

  • J. Kim; L. M. Sayre “Kinetics and mechanism of quinone-mediated amine oxidations” The Local ACS Meeting, Cleveland, OH, March 1998.
  • J. Kim; L. M. Sayre “Studies on quinone-mediated amine oxidations” The Colloquium for Alpha Chi Sigma at Case Western Reserve University, Cleveland, OH, September 2000.
  • K. Ling; J. Kim; L. M. Sayre “Catalytic turnover of benzylamine by a model for the lysine tyrosylquinone (LTQ) cofactor of lysyl oxidase” 222nd ACS National Meeting, Chicago, IL, August 2001
  • J. Kim; D. P. Drontle; C. R. Wagner “Investigation of the intracellular metabolism of nucleoside phosphoramidates of AZT” 40th Annual MIKI Meeting, Chicago, IL, April 2002.
  • D. P. Drontle; J. Kim; C. R. Wagner “Intracellular Metabolism of AZT Phosphoramidates: Metabolism Studies Utilizing 31P NMR” The Great Lake Regional ACS meeting, Minneapolis, MN, June 2002.
  • D. P. Drontle; J. Kim; C. R. Wagner “Intracellular metabolism of AZT phosphoramidates: Mechanistic studies utilizing 31P NMR” 15th International Round Table: Nucleosides, nucleotides and nucleic acids at Leuven, Belgium, September 2002.
  • J. Kim; C. R. Wagner “Studies of the intracellular metabolism of nucleoside amino acid phosphoramidates” 16th International Conference on Antiviral Research at Savannah, GA, May 2003.