My research interests are focused on gaining a better understanding of plant physiological, biochemical and molecular responses to environmental stresses. I am interested in abiotic stresses, particularly heat, salinity, osmotic, drought and oxidative stresses. Using molecular tools, my lab has the opportunity to identify and characterize genes involved in stress responses. Concomitantly, my lab measures physiological performance and biochemical parameters of Arabidopsis thaliana and Saccharomyces cerevisiae exposed to widely varying environments.
In addition, I am interested in studying different aspects of plant mineral nutrition specifically:
-How management of nitrogenous fertilizers would affect plant nitrate transport and nitrogen metabolism?
-How certain metabolites may affect potassium uptake and accumulation in plants?
2008-09. Acquisition of a Microarray Scanner and Real-Time PCR System for Interdisciplinary Research and Teaching in an Undergraduate College Setting. Submitted to National Science Foundation, Major Research Instrumentation Program.(Co-PI with M. J. Kovach,, E. A. Carver, H. G. Spratt, J. Kim.
Cao J, Barbosa JM, Singh NK, Locy RD. (2013). GABA transaminases from Saccharomyces cerevisiae and Arabidopsis thaliana complement function in cytosol and mitochondria. Yeast 30(7): 279–289.
Cao J, Barbosa JM, Singh NK, Locy RD. (2013). GABA shunt mediates thermotolerance in Saccharomyces cerevisiae by reducing reactive oxygen production. Yeast 30(4): 129-144.
Barbosa, J.M.; Singh, N. K.; Cherry, J. H. ; Locy, R. D. (2010). Nitrate uptake and utilization is modulated by exogenous γ– aminobutyric acid, In Arabidopsis thaliana seedlings. Plant Physiol and Biochem 48(6): 443 - 45.
Sang, Y., Barbosa, J. M., Wu, H., Locy, R.D., Singh, N.K. (2007). Identification of a Pyridoxine (pyridoxamine) 5' Phosphate Oxidase from Arabidopsis thaliana. FEBS Letters, Volume 581, Issue 3, 6 February 2007, Pages 344-348.
Barbosa, J.M.; Locy, R. D.; Barger, T. W.; Singh, N. K. Cherry, J. H. (2000). GABA Increase the Rate of Nitrate Uptake an Utilization in Arabidopsis Roots. In Biochemical and Cellular Mechanisms of Stress Tolerance in Plants. Edited by J. H. Cherry. Springer-Verlap. New York, NY. 2000.