Microbial survival is dependent upon adaptation to the surrounding environment. I am interested in those responses occurring in microbial membranes that involve conserved microbial structures, such as phospholipids and lipid A. These membrane constituents can be modified in a variety of ways depending upon external environmental stimuli or simply by nutrient availability. Lipids of various types and origins can be encountered by microbes and used as signaling molecules, membrane building blocks, or energy sources. Projects in my Iaboratory currently involve surveying the lipid content of several bacteria of medical importance in response to environmental and host-specific conditions. I am also interested in extending my research towards aquatic microorganisms of medical and ecological importance.
Madsen, J. A., H. Xu, M. R. Robinson, A. P. Horton, J. B. Shaw, D. K. Giles, T. S. Kaoud, K. N. Dalby, M. S. Trent and J. S. Brodbelt. 2013. High-Throughput Database Search and Large-Scale Negative Polarity LC-MS/MS with Ultraviolet Photodissociation for Complex Proteomic Samples. Molecular and Cellular Proteomics. In press.
Pride, A. C., C. M. Herrera, Z. Guan, D. K. Giles and M. S. Trent. 2013. The outer surface lipoprotein VolA mediates utilization of exogenous lipids by Vibrio cholerae. mBio 4: e00305-13.
Needham, B. D., S. M. Carroll, D. K. Giles, G. Georgiou, M. Whiteley and M. S. Trent. 2013. Modulating the innate immune response by combinatorial engineering of endotoxin. Proceedings of the National Academy of Sciences USA. 110: 1464-1449.
Cullen, T. W., D. R. Hendrixson, D. K. Giles, R. I. Hobb, J. O’Brian, S. A. Thompson, J. S. Brodbelt and M. S. Trent. 2013. EptC of Campylobacter jejuni mediates phenotypes involved in host interactions and virulence. Infection and Immunity 81: 430-440.
Maue, A., K. Mohawk, D. K. Giles, F. Poly, C. P. Ewing, Y. Jiao, G. Lee, Z. Ma, M. A. Monteiro, C. L. Hill, J. S. Ferderber, C. K. Porter, M. S. Trent, and P. Guerry. 2013. The polysaccharide capsule of Campylobacter jejuni 81-176 modulates the host immune response. Infection and Immunity 81: 665-672.
Hankins, J. V., J. A. Madsen, D. K. Giles, J. S. Brodbelt and M. S. Trent. 2012. Amino acid addition to Vibrio cholerae LPS establishes a link between surface remodeling in Gram negative and Gram positive bacteria. Proceedings of the National Academy of Sciences USA 109: 8722-8727.
Cullen, T. W., D. K. Giles, L. N. Wolf, C. Ecobichon, I. G. Boneca, and M. S. Trent. 2011. Helicobacter pylori versus the host: Remodeling of the bacterial outer membrane is required for survival in the gastric mucosa. PLoS Pathogens 7: e1002454.
Hankins, J. V., J. A. Madsen, D. K. Giles, B. M. Childers, K. E. Klose, J. S. Brodbelt, and M. S. Trent. 2011. Elucidation of a novel Vibrio cholerae lipid A secondary hydroxyl-acyltransferase and its role in pathogenesis. Molecular Microbiology 81: 1313-1329.
Giles, D.K., J. V. Hankins, Z. Guan, and M.S.Trent. 2011. Remodeling of the Vibrio cholerae membrane by incorporation of exogenous fatty acids from host and aquatic environments. Molecular Microbiology 79: 716-728.
Giles, D. K., and P. B. Wyrick. 2008. Trafficking of chlamydial antigens to the endoplasmic reticulum of infected epithelial cells. Microbes and Infection 10: 1494-1503.
LaRue, R. W., B. D. Dill, D. K. Giles, J. D. Whittimore, and J. E. Raulston. 2007. Chlamydial Hsp60-2 is iron-responsive in Chlamydia trachomatis serovar E-infected human endometrial epithelial cells in vitro. Infection and Immunity 75: 2374-2380.
Giles, D. K., J. D. Whittimore, R. W. LaRue, J. E. Raulston, and P. B. Wyrick 2006. Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion. Microbes and Infection 8: 1579-1591.